David A. Fruman
Signal Transduction in Lymphocytes
 
Microarray Data
 
Research
Publications
Lab People
Links
Contact
Home
Experiment Design:

Splenic T cells from wildtype or p85b deficient mice were either purified directly ex vivo (termed unstimulated) or stimulated with 2mg/ml of soluble anti-CD3 (clone 2C11) and 50 u/ml of IL-2 for 60 hours. Live T cells were purified from the other splenocytes at the end of stimulation, and mRNA was harvested for microarray analysis, all described more fully in Materials and Methods of the published manuscript. The data contained in the .xls file are d-CHIP filtered values (www.dchip.org) of 12Affymetrix Murine Genome U74Av2 GeneChips.

 
Data Annotation:

Genbank ID: the name of the Gene as found on Genbank (www.ncbi.nlm.nih.gov).

Gene Title: Affymetrix’s description of the gene, if known.

Gene Symbol: Affymetrix’s abbreviated title of the gene.

Affy ID: The ID of the probe set in question, as found on the U76Av2 chip.

WT(Unstim)-1, -2, -3: Wild-type T cells at time 0, three replicates.

KO (Unstim)-1, -2, -3: Knockout T cells at time 0, three replicates.

WT(Stim)-1, -2, -3: Wild-type T cells at 60 hrs, three replicates.

KO (Stim)-1, -2, -3: Knockout T cells at 60 hrs, three replicates.

 
Data File:
  [Splenic T cells +/- anti-CD3 (wild-type vs. PI3K-p85beta-deficient)]
 
 
   
 
 
   
   
Last Updated: June 21, 2004